Filamentous DNA Phages Single Stranded RNA Phages

Filamentous DNA Phages Single Stranded RNA Phages INTRODUCTION: Bacteriophages are one of the most widely studied organisms than that of bacteria and any other living organism. They are mainly used in that of molecular biology studies, because of lesser complexity they are usually preferred over other organism. A bateriophage is a bacterial parasite which can neither grow or replicate on its own but can do so within a given organism. The major success of bacteriophage truly depends upon the genetic element which is usually packed within the capsid. the genetic element can either be a single stranded or double stranded linear,circular DNA /RNA. The filamentous bacteriophages are single stranded DNA phages about 5.5nm in diameter and they usually are present in a ring like form. They show the presence of about 10000 nucleotide and they usually harbour in Gram negative host. They usually adsorb on the tail like component called sex pilus which is responsible for the transmission of plasmids. The major properties of these virus are that it does not harm the host. The other general properties of filamentous DNA phages are as follows: They can be grown in extremely high titre because they do not lyse the cells. They are extremely small in size and they can mainly be studied and used in molecular biology experiments. It is the only virus which has been crystallized for the analysis using x-ray diffraction and a good model for studying mutations. The interaction between the host and the virus can be used as a model for studying characteristics property of oncogenic virus. Three filamentous E.coli phages i.e M13,fd, f1 have been carefully studied and each of them have a life cycle similar to that à ¢Ã‚ ±Ã‚ ·174. M13 bacteriophage: M13 is a filamentous bacteriophage it has circular ssDNA. It contains about 6407 nucleotides. It contains major coat protein P8 and it capped with 5 minor protein P9,P6,P3.P3 protein attaches to the tip of the pilus E.coli. They are non-lethal and they form turbid plaques in Ec.oli they bring about reduction in the cell growth process. They are also used for structural studies. The phage particles: The phage is assembled by that of 50 amino acids protein called p8.It takes about 2700 copies of p8 to make about the coat 90 nm long.It has been seen that is quite flexible .Other distinct proteins which are usually present P9 mainly comprises about 5 copies. P7 p9 are usually seen in micrographs they are extremely small in size containing about 32-33 amono acids.The other end of the phage particles contain about 5 copies of the surface exposed p3and less exposed accessory protein p6.They form a rounded tip and the p6 is the the final protein to interact with the E.coli host during invasion. Phage life cycle: The filament phage uses the F pilus to infect E.coli with the M 13 tip containing the TolA protein on bacterial pilus. The phage genome is transferred to the cytoplasm of E.coli where the resident protein convert single stranded DNA genome to a double stranded replicative form(RF).This DNA acts like a template for the expression of phage gene. The second phase mainly involves amplification of the genome p2 it nicks the double strand form of the genome to initiate the replication of strand without which no replication occurs. Host enzymes replicate the(+) strand resulting in more copies of dsDNA.p5 competes with ds DNA formation by sequestering copies of the (+) stranded DNA into a protein/DNA complex designed to pack recovery phages. Another important protein p10 is important for regulating the number of ds genome in the bacterial host without which no strand an accommodate .Phage maturation requires p4 translational restart p11.Multiple copies of p4 assemble in outer membrane into a table barrel shaped structure.A number of p1 and p11 5/6 copies each)assemble inside the inner bacterial membrane.p1 p11 ans p4 together they are responsible for the release of mature phage. REPLICATION IN M13: Replication in m13 can be explained as follows; Viral (+) strand DNA enterscytoplasm Complementary (-) strandis synthesized by bacterial enzymes DNA Gyrase, atype II topoisomerase, acts ondouble-stranded DNAand catalyzes formation ofnegative supercoilsin double-stranded DNA Final product is parental replicative form (RF) DNA A phage protein, pII, nicks the (+) strand in the RF 3-hydroxyl acts as a primer in the creation of new viral strand pII circulizes displaced viral (+) strand DNA Pool of progeny double-stranded RF molecules produced Negative strand of RF is template of transcription mRNAs are translated into the phage proteins Phage proteins in the cytoplasm are pII, pX, and pV, and they are part of the replication process of DNA. The other phage proteins are synthesized and inserted into the cytoplasmic or outer membranes. pV dimers bind newly synthesized single-stranded DNA and prevent conversion to RF DNA RF DNA synthesis continues and amount of pV reaches critical concentration DNA replication switches to synthesis of single-stranded (+) viral DNA pV-DNA structures from about 800nm long and 8nm in diameter pV-DNA complex is substrate in phage assembly reaction. Research: SINGLE STRANDED RNA PHAGES: Single stranded RNA are found everywhere E. coli lives in the intestinal tract of man and animals. They can also be found in sewage samples .They are harmless creature in case of humans.RNA phages are tailless, icosahedron like that of à Ã¢â‚¬ ¢174 and it mainly involves E. coli phages f2,R17,MS-2 QÃŽ ². The RNA is a linear single stranded molecule having about 3600 nucleotides it contains 3 genes encoding a coat protein an attachment protein (A) and a RNA replicase. The RNA molecule acts both like a template and also like an mRNA so they do not possess a DNA polymerase and the regulation occurs at the translational level. They have large burst size and they form huge crystalline arrays within each bacterium. Cell lysis usually takes place at a time ranging from 30-60 minutes after infection. Virus structure: Virions contain about 180 copies of the coat protein arranged in a T=3 icosahedral shell that encloses THE RNA. alloleviviruses contain about 15 copies of the read through protein.the encapsidated RNA is resistant to ribonuclease treatment.The damaged virion are exposed to RNAase activity.The structures have been resolved by x-ray diffraction methods Life cycle of RNA PHAGE: ENTRY: Upon entry the ribosome they usually attach to the CP gene which is the centremost gene of the RNA molecule. TRANSLATION : The stem and loop structures in RNA block the ribosomal binding sites of the A and the Rep proteins. The translation of the CP gene opens the binding site of the Rep proteins .Both proteins are made but the increasing amounts of the CP proteins bind to the rep site and block translation of the rep gene. About 2 x 10 copies are needed as structural components whereas replicase is needed only in catalytic amounts. Replication of the phage RNA:The QÃŽ ²has a tetramer consisting of one Rep molecule with about three proteins-EF-Ts EF-Tutranslation factors needed for the placement of charged TrnaMOLECULES Synthesis of A protein: The binding site for the A protein is never available on the free(+)strand. The replication begins at the 3’teerminus close to the A gene. After synthesis of the (+) strand begind there is a brief period when the protein A gets translated. The A number is maintained and it is equal to the number of the (+) strand .it is believed that the A protein bring about the interaction of the RNA with the CP molecules PARTICLE ASSEMBLY: THE CP PROTEIN MOLE CULES spontaneously aggregate around the newly synthesized (+) strand and form an icosahedral shell. Cell lysis: Once about 10000 phages are formed the cell undergoes lysis and no lytic enzymes are left behind ,the mechanism still remains unknown. The QÃŽ ² phage: It is an RNA virus affecting E.coli. Contains a positive sense single strand RNA. It is one of the smallest known viruses measuring about 24 nm in diameter. Its icosahedral capsid consists of about 180 copies of the same protein. The strands acts like that of an mRNA and it also acts like a template for the replication of a complimentary strand. REFERENCES: Filamentous Bacterial Viruses(BACTERIOLOGICAL REVIEWS JUNE 1969 pg 177-209.ASM PRESS THE BACTERIOPHAGES 2ND ,BY CALENDER EDITION OXFORD PRESS MOLECULAR BIOLOGY BY FREIFIELDER FROM THE WEB

Competition Level Between Shanghai Port and Busan Port

Shanghai Port Is located In the front edge of the Yawning River Delta and It becomes the first busiest container port In the world whereas Bushman Port which Is located In the southern part of the Korean peninsula ranked the fifth. Both ports handle approximately 90% of the total container throughput, and they are also the major hubs of the seaborne transportation in the world (World Port Source AAA, World Port Source Bibb).This essay will discuss the level of competition between Bushman Port and Shanghai Port, and identify the external factors that should be considered by both ports' management in developing their strategic development plans. Level of competition The late growth of China on a global scale in recent years has affected on both Bushman Port and Shanghai Port In several levels of competition. There are three key competitions between them which are geographical positioning, transshipment capableness and low comparative costs.In terms of geographical positioning, Bushman Port has become an appropriate choice due to Its location between Japan and China. It has mm water depth and planning to Increase up to mm In 2020, and Its world class infrastructure allows the calling of mega large vessels. Furthermore, Bushman Port has 41 container berths and systematic connections for global logistics corporations to help save costs and time, so it can act as a feeder hub aiming for small ports for a majority of North-East Asian transshipment carriers.For example, inland transport in Northern China will be expensive and inconvenient, thus exporters or importers choose to transport their shipments to Bushman Port first then Shanghai Port (BAA 013). On the other hand, China created Shanghai as a finance shipping hub because Shanghai Is a port city. It became an international maritime capital because It focused on economic Instead of political logic (BAA 2013). Similarly, both Shanghai Port and Bushman Port are part of the mall trunk line functioning as hub port for relative countries, but Shanghai Port has the ambition to challenge Bushman Port as the main hub port of Northeast Asia.This Is because the growth of container volume In China ports has been fuelled by the decision of many multinational companies to locate production of a whole range of consumer goods from their domestic plants, mainly in USA and Europe, to China where production costs are much cheaper (Park, Anderson & Choc 2006). However, the inconsistent weather of China has benefited South Korea. For instance, ports in China are forced to close for up to two weeks of each calendar year due to fog and stormy weather.Moreover, Shanghai Port could not compete with Bushman Port deep water harbor, causing It failed to attract mega huge vessels from big corporation to call (Shepherd 2011). Regarding to reinstatement capabilities, Shepherd (2011) stated that the newly extended feeder network of Bushman Port connects with over sixty others ports In China, five In Russia, and thirty In Japan, making Its as the chosen destination for huge vessels and to transshipment cargo. Conversely, the development of Hangs New Port which is traffic and establishing transshipment capabilities comparing to its old Wigwagging container terminal.Moreover, Shanghai Port is improving its customs clearance procedure and cargo handling system to attract more transshipment cargo (McKinney 2011). Consequently, both ports have similar transshipment capabilities which are also the main competitiveness between them. Towards low comparative costs, in order to attract more transshipment cargo, Bushman Port promoted ‘Free Trade Zone', reducing port tariffs, and exempting 100% of Transshipment Entrance Fee since 2003 as the marketing strategies to attract foreign investment and compete with Shanghai Port (Park, Anderson and Choc 2006). For example, the exemption of the $2. 0 for 1 TEE entrance fee could reduce the $2,200 fee for a vessel carrying 1,000 TIES, and it also provides exclusive berths for coastal shipping companies and offers volume incentives to secure more cargo volumes (BAA 2013). On the other hand, Shanghai Port utilized a Volume Incentive System for shipping companies when the transportation volume is increased by more than 20% compared with the previous year. In addition, the Shanghai Port has also been trying to improve competitiveness by discounting port tariff in the Hangs New Port, and plan to establish logistics Free Trade Zone in the hinterland (Park, Anderson and Choc 2006).External factors in developing strategic plans There are several external factors that should be considered by Bushman Port and Shanghai Port in developing their strategies development plans. The first factor is the government involvement. According to Lee, Lee, Gang & Lee (201 1), port management had changed rapidly in recent years due to the structural changes in international trade and sea transport, so government plays an important role to cope with the changes of envi ronment.The establishment of co-petition and global network between competitors will generate positive impact on efficiency and access of the port management strategies with the special government support (Shepherd 2011). In addition, government can encourage shipping companies to form alliances where they can connect their transport system hence improving services. Moreover, the supplementary government supports with positive impact on efficiency, credibility, and competitiveness of the port management strategies could lead Bushman Port to have greater impact function as a Northeast Sais's hub-Port (McKinney 2011).Additionally, the advancement of government policies are followed by the changes made in the environment of port management. How government involves in port management will impact on the port's future developing of plans. In Shanghai, the central government had not implicated in the ownership of ports, but had got involved in an oversight role for strategic planning. For example, local authority had rights to undertake the ports planning, but still all plans had to be approved by central government (Xx 2007). This cooperation between government and port management will lead to improvement of international trade.Furthermore, comply with the Port Laws, it can encourage foreign investment as to build an attractive business environment in Shanghai Port, presenting Shanghai Port as an International Shipping Centre (SC) (McKinney 2011). Secondly, the growth of ports in the world had been affected due to the global economic downturn in 2012. Negative economic growth will not only put current projects at risks, but also the future project. This will the government to have less revenues and resources (Waters 2012). Therefore, the government should step in and take control of the situation.Government needs to prepare the country to become a low cost and high productivity place to invest which then attracts foreign investments, helping the country to generate profits. For example, government could implement strategies, such as education and direct investment (FED) to promote the local economy for future development of ports (Port Technology 2013). The investment in human capital or education can produce workers with better knowledge, and the productivity will increase when workers have more capabilities.Moreover, governments can send port employees to countries like Singapore and Hong Kong to familiarize with their operating system as they are the world most efficient ports. This will improve the port technology and productivity whilst workers return. Furthermore, economic growth can be achieved with the redevelopment of Bushman North Port and the nearer completion of Bushman New Port had as it will create whereas Shanghai Port can also benefited from the increase of national strength in China (Xx 2007). Another factor is the inland transport system.A well developed port should also include efficient inland transport. This is because mul tinational shipping company will normally choose port with speedy and productivity connection between the port and inland transportation to faster deliver cargoes to their customers (ROR, Lanai & Maim 2007). Therefore, both South Korea and China government should increase attention and funding towards the improvement of inland transport system within the port, such as railway network which connect to major cities in the countries.Moreover, the investments in rail and road access could help Bushman Port and Shanghai Port to remain as the major container hubs in the world, and create a swift, simple and cheap choice of transportation to transfer cargo within the countries as well as produce more Jobs for the locals (Shepherd 2011). In addition, governments could also focus on congestion robber especially in the city to allow trucks to transport goods more rapidly. For example, encouraging trucks to operate in certain hours and taking a detour road can help to save time and cost (Lee 2 013).As a result, good connection of inland transport could help Bushman Port and Shanghai Port reduces the problem of congestion, and also allows cargoes could smoothly move through the port. Apart from the previous mentioned factors, environmental pollution is another key external factor that Bushman Port and Shanghai Port have to consider in developing their strategic development Lana. There are several causes of the port environmental pollution. For instance, diesel engines at ports, trucks, trains, and cargo handling equipment, creating a lot of air pollution that has affected the health of port workers, and residents living in the communities.In addition, major ports especially Shanghai operating around residential areas or city, causing both workers and residents to face extraordinarily high health risks which related to air pollution. According from the human epidemiological and California studies, diesel exhaust increases risks of cancer, and approximately 70% of the cancer risk is caused by air pollution (NRC 2004). Furthermore, the dredging berth and deepening access channels of Shanghai Port and Bushman Port in order to accommodate large vessels in the future are causing water pollution.It not only damages the water quality, marine life and ecosystems, traffic Jams, and could be loud, ugly and brightly lit at night. These effects also pose negative health effects to human. For instance, noise pollution has been linked to hearing impairment, high blood pressure, lack of sleep, performance reduction, and even aggression behavior. Bright lights at night and the flashing lights of straddle carries and forklifts could influence surrounding residents, leading stress and irritation (NRC 2004).Consequently, Bushman Port and Shanghai Port have to do their best to avoid, to prevent, to control, and to measure the environmental pollution which caused by several port operations, with the ultimate aim to become a green port. Conclusion In conclusion, Bushman Po rt and Shanghai Port have their own respective advantages to compete with each other in several aspects in order to gain competitive advantages in the marine industry. However, there are several external factors that Bushman Port and Shanghai Port have to take into consideration while developing their strategic development plans in order to avoid the disadvantages to them.

Pennsylvania Colleges and Universities Pursuing Online and Campus Based Education in Pennsylvania 2019

With 49 Fortune 500 companies based in the state, working professionals who head back to school in Pennsylvania have an abundance of career options. Pennsylvania colleges and universities are training students to work in the commercial and industrial centers of Pittsburgh and Philadelphia. College degree programs and career options throughout the Keystone State of the Northeast are more plentiful than ever before. Graduates of Pennsylvania Colleges and Universities have Best Opportunities with Local Employers Pennsylvania college graduates are prepared to effectively compete for local positions. Some of Pennsylvanias top employers include: FedEx Ground headquartered in Pennsylvania. General Nutrition Centers headquartered in Pennsylvania. Entercom Communications headquartered in Pennsylvania. Kappa Publishing Group headquartered in Pennsylvania. Mellon Financial headquartered in Pennsylvania. PNC Financial Services headquartered in Pennsylvania. Pennsylvania Real Estate Investment Trust headquartered in Pennsylvania. Pennsylvania Colleges and Universities help Working Professionals Thrive in a Strong Local Economy With an unemployment rate and median household income on par with the national average, Pennsylvania offers a stable economy for working professionals with a college degree. Philadelphia shipyards and seaports on the Delaware River continually boost the Pennsylvania economy. The most recent statistics from the U.S. Census Bureau and Department of Labor reveal: The 2017 Pennsylvania gross state product was $430 billion. The September, 2017 Pennsylvania unemployment rate was 4.1%, equal to the national average. 5,999,600 people are employed in Pennsylvania. The 2016 2017 Pennsylvania median household income was $45,941, on par with the national average. .u133d2f8af424288a34b3564b005ee503 { padding:0px; margin: 0; padding-top:1em!important; padding-bottom:1em!important; width:100%; display: block; font-weight:bold; background-color:#eaeaea; border:0!important; border-left:4px solid #34495E!important; box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -moz-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -o-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -webkit-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); text-decoration:none; } .u133d2f8af424288a34b3564b005ee503:active, .u133d2f8af424288a34b3564b005ee503:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; text-decoration:none; } .u133d2f8af424288a34b3564b005ee503 { transition: background-color 250ms; webkit-transition: background-color 250ms; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; } .u133d2f8af424288a34b3564b005ee503 .ctaText { font-weight:bold; color:inherit; text-decoration:none; font-size: 16px; } .u133d2f8af424288a34b3564b005ee503 .post Title { color:#000000; text-decoration: underline!important; font-size: 16px; } .u133d2f8af424288a34b3564b005ee503:hover .postTitle { text-decoration: underline!important; } READ 10 Job Search Rules You Need to BreakStudents have Access to Flexible Programs at Pennsylvania Universities and Colleges Pennsylvania universities throughout the state are now offering flexible degree programs that allow students to maintain work and personal responsibilities. Unique combinations of campus-based and online education programs are now available through Pennsylvania schools, such as: Kaplan University: Broomall College Campus, Southampton University Campus, Pittsburgh College Campus, Philadelphia University Campus, and Online Programs. University of Phoenix: Harrisburg University Campus, Philadelphia College Campus, Pittsburgh University Campus, and Online Programs. ITT Technical Institute: Harrisburg College Campus, Pittsburgh University Campus, Monroeville College Campus, King of Prussia University Campus, Bensalem College Campus, Dunmore University Campus, and Online Programs. For more links to Pennsylvania colleges and universities, students are encouraged to visit College-Pages.com, the leading education and career resource website. Students will also find access to informative articles on making education and career decisions in the state of Pennsylvania. .u17506b6a01514f9db0569d7496f66ec0 { padding:0px; margin: 0; padding-top:1em!important; padding-bottom:1em!important; width:100%; display: block; font-weight:bold; background-color:#eaeaea; border:0!important; border-left:4px solid #34495E!important; box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -moz-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -o-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); -webkit-box-shadow: 0 1px 2px rgba(0, 0, 0, 0.17); text-decoration:none; } .u17506b6a01514f9db0569d7496f66ec0:active, .u17506b6a01514f9db0569d7496f66ec0:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; text-decoration:none; } .u17506b6a01514f9db0569d7496f66ec0 { transition: background-color 250ms; webkit-transition: background-color 250ms; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; } .u17506b6a01514f9db0569d7496f66ec0 .ctaText { font-weight:bold; color:inherit; text-decoration:none; font-size: 16px; } .u17506b6a01514f9db0569d7496f66ec0 .post Title { color:#000000; text-decoration: underline!important; font-size: 16px; } .u17506b6a01514f9db0569d7496f66ec0:hover .postTitle { text-decoration: underline!important; } READ West Virginia Colleges and Universities Pursuing Online and Campus Based EducationRelated ArticlesDelaware Colleges and Universities Pursuing Online and Campus-based EducationNew Jersey Colleges and Universities Pursuing Online and Campus Based Education in New JerseyArizona Colleges and Universities Pursing Advanced Education in The Grand Canyon StateUtah Colleges and Universities Pursuing Online and Campus Based Education in Utah, the Beehive StateIowa Colleges and Universities Pursuing Online and Campus-based Education in Iowa, the Hawkeye StateNorth Dakota Colleges and Universities Pursuing Online and Campus Based Education in North Dakota

Homemade Mosquito Repellents That Work

Have you made or tried a natural homemade mosquito repellent? If so, let other readers who whether it was effective for you. Do you have any favorite natural mosquito repellent recipes? Lemon Juice Lemon juice worked really well for me, and Im a mosquito magnet. I had shorts on and put lemon juice (just normal lemon juice you can buy from the store) on my legs (it wasnt sticky) and the mosquitoes stopped biting my legs. Unfortunately, I didnt have a sprayer and they moved on to biting me through my shirt; but not my legs though! — Charlie Wow Vicks Really So simple an I think it works!! Just tried it this morning and no bites (yet! Knock on wood!) I was mixing up all these spices from the kitchen then came across that post and thank goodness I did! I just moved back to Orlando and the Mosquitos here are only getting worse!! I havent been going outside. I think and hope u solved my problem!!!! Thank u!!! — Flsnowbunnyy Mr Clean please stop! I would like to ask Guest Mr Clean to re-consider applying dryer softener sheets to his and his childrens skin. Chemicals in the sheets might absorbed through the skin and into the bloodstream, possibly affecting the brain or other vital organs. With all due respect, it just doesnt seem safe. —NatureHealth Dawn I set out a container with water near patio. When larva appear I add a drop of two of Dawn. I have also caught adult mosquitoes. This seem to reduce the overall population in the area. I keep a ceiling fan on high when on the patio and the mosquitoes stay away. I suspect the fan dissipates the CO2 from my breath that attracts them. —Guest Donald Natural Mosquito repellent!! Rub a light layer of vicks rub on the bare skin! Not only will you be able to breathe well... little pesky boogers stay away!!! :) —VICKS GOTTA TRY IT CHEAP AND EFFECTIVE Ive been using regular bounty dryer sheets for about a year now on my self as well as my 6 yr old, 4 yr old and 1 yr old, and it works AMAZINGLY!!! Just rub it on and thats it! I even tape them to the inside of my tent while camping and Ive killed one maybee 2 sqeeters. —Mr. Clean Vinegar of the Four Thieves This stuff kept away the Bubonic Plague! Seriously strong but natural and cheaper than oils. Smells foul when wet but OK when dry: Vinegar of the Four Thieves Insect Repellent Ingredients: ââ€"  1 32 ounce bottle of Apple Cider Vinegar ââ€"  2 TBSP each of dried Sage, Rosemary, Lavender, Thyme and Mint ââ€"  At least quart size glass jar with airtight lid How to Make the Vinegar of the Four Thieves Insect Repellent: 1.Put the vinegar and dried herbs into large glass jar. 2.Seal tightly and store on counter or place you will see it daily. Shake well each day for 2-3 weeks. 3.After 2-3 weeks, strain the herbs out and store in spray bottles or tincture bottles, preferably in fridge. 4.To use on skin, dilute to half with water in a spray bottle and use as needed. 5.Use whenever you need serious bug control! [Note: This mixture is very strong and has antiviral and antibacterial properties.] —Normski It works I take cinnamon pills. I have not been bitten in over 2 years. —twice shy Mosquito repellent fumes Mixture of dried leaves and fruit coats of tree Pongamia Pinneta with 2 % Neem oil and 2 % Pongamia Pinneta oil when burned like incense sticks to give fumes repels mosquitoes from the room. Pongamia Pinneta is called KARANJ in Hindi. —Sushil Kumar Sharma Natural Room Spray Gday! A while ago my Mum found a great add in the paper with how to make this, we went and bought all the essential oils and carrier oil which cost a fortune at the time we figured, we were getting our moneys worth if it worked, which it did !It was great! Now I want to make it up, Mum cant find the list of ingredients! sigh, all I can remember, is it had metholated spirits and water with it, vinegar and about 8 or more 100% pure oils, you would literally see the flies come in and back off where she had sprayed in the kitchen, can anyone help here? I possibley have some of the ingredients wrong and it was in a spray bottle. I do know of a mix to deter toads though, its affectionately known by my friends who invented it as 8 hops cos thats about as far as they get after being sprayed by it, I spray around my doorways and sliding doors, to prevent them coming into the house. Its half and half of Metholated Spirits and Dettol, dont use cheap brands they stink. —Tonianne repellents citronella is the best! try it now please —adrian Mosquitoes repellents I have try inside my apartament, the little patio and entrance putting a glass gar with water and smash garlic, it seams that have worked on me. You can try that. —Margie SantaMaria lavender oil i usually get tons of mosquito bites when i go camping but the last couple of times i went i used lavender oil as a mosquito repellent and it worked amazingly! i didnt get a single bite! —crystal vinegar I was told that regular vinegar would repel them but it didnt. i also tried that thing you clip on and it didnt work either. Anyone have any experience with those two? my friend tried the vinegar and it worked for her. and people swear by the clip on thingy. i wonder why they still attack me. —sharon Skin So Soft has DEET Avon Skin So Soft is an effective repellent because it contains DEET. And DEET is less expensive than the Avon product... —Kathryn CATNIP OIL is HATED by mosquitos! Cant believe you didnt mention the essential oil of Catnip! It is off the charts for keeping mosquitos away, AND it doesnt stink! One way you can mix essential oils into water based products is to mix Polysorbate 20 with your essential oil 1:1 BEFORE mixing into water or vinegar..Basically the polysorbate is a naturally derived emulsifier. It will make your oil mix into water so it is equally dispersed, say, in a sprayer. I buy my catnip and polysorbate 20 from a small essential oil distributer. Prob cant name it here, but just google it. You can mix in Witch Hazel instead of water or vinegar or just use all three. —gina castor oil is NOT an essential oil Castor oil is a carrier oil, but I would not recommend it for an application like this since it is very thick and sticky. Two other EOs which are mosquito repellents are Lemongrass and Lavender. —Erin retired chem teacher liquid Ivory soap for dishes worked very well with the added benefit of being able to clean dishes. I rubbed one arm only with it and mosquitoes stayed away for over an hour. My other arm was attacked often. —DHK Listerine and Mosquitoes My little Yorkie gets deathly sick from heartworm medication, cant tolerate most chemicals so can never take her out in evening/early morning. Last year on a tip, tried putting a neckerchief on her sprayed with Listerine...and slapped some on me too (I seem to be a major mosquito magnet). Well, it worked like a charm! Unbelievably, I didnt get a single bite and I live in a huge mosquito infested area d/t lots of woods and ponds. Still dont like taking my little pooch out at night, but if I must take her somewhere, I dont leave home without her Listerine soaked neckachief and I love that there are no harsh chemicals to stress her liver and pancreas (she suffers from chronic pancreatitis so its essential I totally limit chemicals on her). —Judyann J Neem Oil Light up the tip of a wick (4 inch long cotton threads rolled by hand to form a wick) immersed in a small dish containing NEEM )oil (50ml costs Ind.Rs50/- ).The fumes are not harmful.ventilate the room after half hour.the mosquitoes fly away if windows are open.The just DROP DEAD if this lighted lamp is kept in a closed room. I have tried it and it really works. for outdoors, you can rub neem oil on the exposed part of the body to keep away mosquitoes. —JAYAKUMAR.R Listerine I Have tried this , spraying it full strength on my dogs bed at night. It definately helps reduce the number of mozzies but not all of them. My dog doesnt mind the smell, I used the regular brand listerine. However my friend sprayed her back yard area with the mint listerine and she said it attracted a lot of ants, so she advises againt the sweeter style listerine. —fastpurpleharley Ms. Im such a mosquito magnet (also black biting flies) that my kids say Throw Momma off the deck! In a short trip outside today, I got 4 bites, my husband got none. My skin temp is cooler than most; its not the time of the month (Im past that). Oddly I think Im totally immune to poison ivy--may never have had it. Connection? —Ellen Know your oils! To repond to Keithgvp Cassia Oil is NOT Cinnamon Oil. —Tommy Internal Mosquito Repellant Ive found that taking excessive amounts of Vitamin C for a few days before going camping keeps mosquitos and bugs in general away from me. Being water soluble, Vitamin C wont accumulate in the body, but instead will be excreted in sweat. Bugs hate it! NOTE: If you experience diarrhea youre taking too much. —drdulttl lemon EUCALYPTUS Ok so if you buy lemon eucalyptus as a liquid incense it is far more cheaper than buying the oil, as you can dilute the incense in baby oil or mix it with body lotion apply. And this way it is cheaper and probably better for your skin as with the oil it wasnt really designed for the skin but for oil burners. As well another good technique if you have a baby is to just spray a little on their teddy/doll or something they sleep with. And yes lemon eucalyptus does work (personal experience). —kandii mosquito repellent I hear that spraying oneself and area with the reg. listerine helps get rid of mosquitoes. I hope this helps. —audrey essential oil caution When using any essential oil, mix a small amount first and test it on your wrist. If you have a bad reaction, dont use it! The only oil I have issues with is Lemon. Cinnamon Oil has been a favorite of mine for other uses, so will be trying it as a mosquito repellent tonight. Im guessing the camphor and eucalyptus in Vicks is what does the trick against mosquitos. —Everythingherbal Listerine Dryer Sheets I live in Northern California. We have mosquitoes. I spend a lot of time in the woods and attract the little buggers like crazy. I have found Regular Listerine( buy the cheaper generic brands ) and even clothing softener sheets work very well . I see where the mouth wash contains eucalyptus oil and I prefer it to the sheets . I just use it undiluted in a spray bottle..Apply it every couple of hours. —Jim All Natural Bug Deterrent Candle I make soy wax candles and have been wanting to make a Be Gone Bug one, esp living in the Southeast! Ill let ya know how it turns out... —Heather Jernigan Repellent Alternative I like the idea of making my own insect repellent but I don’t always have the time (or all the ingredients) to do so. I did some research and learned that Oil of Lemon Eucalyptus (OLE) is the only plant based product recommended by the EPA and CDC for use as an insect repellent. (Check out http://www.cdc.gov/ncidod/dvbid/westnile/RepellentUpdates.htm if your interested in what they have to say) OLE is proven to work as well as DEET without the harmful effects. If anyone is interested in trying it, Cutter and Repel both make an OLE product —Ashley mint leaves and vicks can help Ive found Vicks very useful as the mosquito repellent. Whats the annoying chemical in it for mosquitoes? For other insects such as house flies, I found mint leaves dipped in a bowl of water are very effective. —irfana aamir Mosquito repellents I have used Avons repellent also, but I discovered if I get some mosquito fish and put them in water around the outside or see standing water and dump it out it helps keep them down. I know some parts of our country are just mosquito prone. I wish everyone could get some fish that eat this kind of pest. —Anna Elizabeth Wooten vics I found that if I apply Vicks on a few places on my legs, arms and forehead I have no problem the whole night. —AK4Services Dangerous Cinnamon Oil! This is dangerous! I am warning readers not to try cinnamon oil, even diluted 20 to 1, as a mosquito repellent. At that ratio I sprayed some on my face and shoulders (luckily not in my eyes) and in seconds my skin was red and burning. I was in agony as I tried to wash it off, and covered my skin with bicarbonate of soda (a base). I cant believe a PhD would recommend this as a treatment without probably trying it herself. This seems highly irresponsible. Imagine if someone, perhaps a child, sprayed themselves with a cinnamon oil repellent and was nowhere near a source to wash it off? Again, This is a highly dangerous substance: WARNIING, DO NOT USE CINNAMON OIL! (or at the least, the kind I purchased - Chinese Cassia Cinnamon Oil made by a company called Now Foods, in Bloomingdale Il. However, I have had success with a lemon-eucalyptus combination cut with witch hazel. —Keithgvp The Listerine Remedy Ive tried Listerine on and around my doors to keep mosquitoes away. It works just fine. Although you do have to reapply every couple of days, it sure beats spending big $$ on commercial repellents. The old tried and true Skin So Soft (Avon)works well also, in conjunction with one of the oils. Skin so Soft works well on my grandson. But if we are going out for a LONG time, I will lightly spray his clothing BEFORE he is dressed with Off. I just find using a variety of methods is quite efffective. —Mellinpie Listerine for Mosquito control I too have heard of using Listerine around the deck and on the screens and windows. Has anyone else heard of this? If so, how well does it work and why? —James Talley Mosquito Repellent For a couple of backyard events, before guests arrived, we sprayed equal parts of lemon-flavored ammonia mixed with lemon Joy or Dawn (liquid dishwashing soaps) in one of those Miracle Grow hose dispensers, and sprayed around the yard and bushes. This is a temporary fix, but seemed to help. —jandalton Favorite Natural Mosquito Repellents The easiest repellent to make, for me, has been cinnamon oil in olive oil or another vegetable oil. Unlike most of the other mosquito-repelling oils, I actually have cinnamon oil in the cupboard to use as a flavoring for cooking. A little goes a long ways... dont over the cinnamon, especially if you are going to use the product on your skin. If you rub it in your eyes it will burn. My favorite natural mosquito repellent, in terms of effectiveness and scent, is lemon eucalyptus oil in either vodka (non-greasy) or else a mix of vodka and vegetable oil. Another really good natural mosquito repellent is lemon oil. If you arent up for making your own insect repellent, Ive seen lemon oil available commercially as a Burts Bees bath oil. —gemdragon